ABSTRACT
Objective To study the chemical constituents from the Stichopus variegatus .Method The compounds were isolated and purified by silica gel column chromatography ,Sephadex LH-20 gel filtration and recrystallization .The structures of these compounds were identified through chemical reactions and spectral analyses ,such as IR ,1 H ,13C NMR ,MS .Results Three compounds were identified as 3-O-[3-O-methyl-β-D-glucopyranosyl (1→3)-β-D-xylopyranosyl (1→3)-β-D-glucopyranosyl (1→2)-β-D-xylopyranosyl]-holosta-7 ,24-diene-3β,23S-diol ,named as variegatuside C ;3-O-{3-O-methyl-β-D-glucopy-ranosyl(1→3)-β-D-xylopyranosyl(1 → 4)-β-D-glucopyranosyl (1 → 2)[β-D-glucopyranosyl (1 → 4)]-β-D-xylopyranosyl-holosta-8-ene-3β, 23S-diol ,named as variegatuside D ;3-O-{3-O-methyl-β-D-glucopyranosyl(1→3)-β-D-xylopyranosyl(1→4)-β-D-glucopyranosyl (1→ 2 ) [3-O-methyl-β-D-glucopyranosyl (1 → 3 )-β-D-glucopyranosyl (1 → 4 )]-β-D-xylopyranosyl}-holosta-9-ene-3β,23S-diol , named as variegatuside E .Conclusion All three compounds are new compounds .
ABSTRACT
Objective: To study the constituents from the sea cucumber, Stichopus variegatus. Methods: The crude saponins of S. variegatus were desulfated with pyridine-dioxane (1:1), the desulfated products were separated by multi-chromatography to afford two compounds. Results: On the basis of chemical methods and spectral evidences, especially 2D-NMR and ESI-MS, these two compounds were identified as 3-0-[β-D-xylopyranosyl-(1→2)-4′-O- sulfate-β-D-xylopyranosyl]-holosta-9-ene-3β,12α,17α, 25β-tetraol sodium salt, named as variegatuside A (1) and 16β-acetoxy-3-O-β-D-xylopyranosyl-holosta-22,25-epoxy-9-ene-3β, 12α,17α-triol, named as variegatuside B (2). Conclusion: Compounds 1 and 2 are new compounds.